LSBS 5544-0

Brief

生物分子（諸如：蛋白質與DNA）和受體的交互作用是現代生物物理領域的核心研究之一，目前已有多種實驗技術可以量化這些作用 力。本課程將涵蓋各項常用於測量生物分子親和力的實驗技術，旨在讓學生們熟悉這類生物物理實驗技術之原理、設計與應用。

Description

Course keywords: binding interaction, isothermal titration calorimetry, microscale thermophoresis, surface plasma resonance, fluorescence spectrometry 一、課程說明 This course will cover several modern biophysical tools for protein-ligand interactions. These tools include isothermal titration calorimetry (ITC), microscale thermophoresis (MST), surface plasmon resonance (SPR), fluorescence spectrometry, and uv-vis spectrometry with emphasis on the advantages and limitations of each technique. There will be a demo of ITC, MST, and fluorescence spectrometry in class. 二、指定用書 Biophysical Tools for Biologists, Volume One: In Vitro Techniques. Edited by Dr. John J. Correia and Dr. H. William Detrich, III (2008) ISBN: 978-0-12-372520-2 三、參考書籍 1. Principles of Fluorescence Spectroscopy. 3rd edition, Joseph R. Lakowicz (2011) ISBN: 0387312781 2. Handbook of Single Molecule Fluorescence Spectroscopy. Christopher Gell, David Brockwell, Alastair Smith (2006) ISBN: 978-0198529422 四、教學方式 互動式教學，鼓勵學生提問和發表意見。 五、教學進度 March: basic principles of protein-ligand interactions April: ITC and SDC May: SPR; basic principles and applications of fluorescence spectroscopy June: fluorescence single-molecule measurements 六、成績考核 課堂參與(20%)，每週期刊口頭報告(40%)，每週作業(40%) 七、講義位址 http://lms.nthu.edu.tw/ AI 使用規則: 有條件開放，請註明如何使用生成式AI於課程產出

Timetable

Scores

Average Percentage 89.75

Std. Deviation 1.48

Remarks

16週

Restrictions

Compulsory

Elective

Cross Discipline

First Specialization

Second Specialization