BMES 5466-0

Brief

人與人在交換資訊時，除了語言，照片是一個非常有效的訊息傳遞方式。而科學家在進行意見交流時，影像也 往往是最直接有效的溝通方式，每一個重要的生物影像技術發明，大多都獲得諾貝爾獎的肯定。而現今的生物影像技術，正是百花齊放的年代， 從功能性造影到超解析顯微鏡，都讓過去難以觀察的生物現象得已被展示及記錄。本課程除介紹各式生物影像技術之原理及應用，亦將安排帶領 同學實地參觀部分儀器設施，並探討目前技術的瓶頸及解決方式，透過課程及討論，希望帶給學生未來在研究上第一手的最新知識。 When people exchange information，in additìon to language，photos are very effective way to convey information. When scientists exchange opinions, images are often the most direct and effective way of communication. Most important bio-imaging technology inventions have won the Nobe1 Prize. Now is the golden age bio-imaging. From functiona1 imaging to super-resolution microscopy, biological phenomena that were difficu1t to observe in the past now can be easily displayed and recorded. In addi

Description

Course keywords: Bioimaging, microscopy, digital, image analysis, image segmentation Course Description To understand the foundation of modern Digital microscopy. Text Books NaN References: Teaching Methods 2 hours introduction, 1 hour group discussion Syllabus (Might change due to the teaching progress) 1. Bioimaging systems for biological sample 2. Foundation of optical imaging and microscopy 3. Different types of optical microscopy 4. How to build an automated microscopy system-hardware and software 5. How to build an automated microscopy system-hardware and software 6. How to build an automated microscopy system-hardware and software 7 Super-resolution microscopy 8. Electron microscopy 9 Sample for optical microscopy-Flouresent proteins and immunostaining 10. Sample for optical microscopy-Tissue clearing and expansion microscopy 11. Midterm 12. Experiment (sample preparation) 13. Experiment (imaging sample with confocal microscopy) 14. Digital image properties 15. Image viewing and processing with ImageJ 16. Image segmentation and quantitative analysis 17. Deep learning based automated image segmentation. 18. Final. Evaluation Experiment report: 30% Midterm: 35% Final: 35%

Timetable

Scores

Average Percentage 89.09

Std. Deviation 5.9

Remarks

Restrictions

Compulsory

Elective

Cross Discipline

First Specialization

Second Specialization